Heterologous Growth of Sarcoma

Ehrlich was the first to demonstrate, by his famous “zig-zag― (i.e., mouse to rat to mouse, etc.) experiment, that the Ehrlich mouse sarcoma can grow in rats (4). Russell showed that a mouse tumor designated as “@7― multiplied and survived in rats 9 days before regressing (1@). Murphy demonstrated that mammalian tumors may be grown in the developing chick embryo (7). Mur phy and Rous reported that rat tumor pulp in jected through the uterine wall into the rat em bryo, a few days prior to the end of pregnancy, became established but did not grow.' It is diffi cult to carry out this procedure without bringing gestation to an end (8). Bullock, using rats less than @4hours old, succeeded in growing mouse Carcinoma 63 for 14 days and mouse Carcinoma @2O6for 10 days before regression took place. Mouse Sarcoma 37 was maintained 49 days, with 5 passages, in baby rats (8). Gheorghiu, using rats not more than 3 hours old, was able to transplant a mouse carcinoma serially for @Opassages. The tumor regressed after growing 14 days in the first passage. Its longest period of growth occurred in the twentieth passage, where it was maintained for @28 days (5). In all these experiments the tumors eventually regressed. Murphy and Sturm (9) successfully transplant ed mouse sarcoma into the brain of rats, guinea pigs, and pigeons and confirmed Shirai's finding (IS) that the brain may be used for heterologous transplantation. Nagayo (10) transplanted sugar induced spindle-cell mouse sarcoma serially in adult rats for ten generations. This procedure did not change the viability of the tumor cells, and there was no significant alteration of the tumor morphology. Putnoky (11) claimed to have grown the Ehrlich mouse sarcoma in lactic acid—treated adult rats for @6passages. Greene (6), using the anterior chamber of the eye, has done extensive